Gender Differences in Ischemic Cardiomyopathy. You, H. et al. Chinigarzadeh, A., Muniandy, S. & Salleh, N. Estrogen, progesterone, and genistein differentially regulate levels of expression of alpha-, beta-, and gamma-epithelial sodium channel (ENaC) and alpha-sodium potassium pump (Na(+)/K(+)-ATPase) in the uteri of sex steroid-deficient rats. Stachenfeld, N. S. Sex hormone effects on body fluid regulation. X.Z., Y.G., A.A.S.B., J.Z., Y.S., H.S. The expression levels of α-ENaC, derlin-1, AMPK, and related molecules were also examined by western blotting and real-time PCR in cultured mouse renal collecting duct (mpkCCDc14) epithelial cells following estrogen treatment. This inhibition is caused by a decrease in the number of osteoblasts secondary to a shift in the differentiation of mesenchymal cells away from the osteoblastic lineage, and an increase in the death of mature osteoblasts. Phosphorylation of Nedd4-2 by Sgk1 regulates epithelial Na(+) channel cell surface expression. This work was supported by the National Natural Science Foundation of China grants 81470040 (X.L. You can also search for this author in Kamynina, E. & Staub, O. You can also search for this author in Liang, X., Butterworth, M. B., Peters, K. W. & Frizzell, R. A. AS160 modulates aldosterone-stimulated epithelial sodium channel forward trafficking.
Water retention occurs when renin-angiotensin, a compound that the kidneys create, is stimulated via excess estrogen circulating throughout the body. prepared the manuscript.The authors declare that they have no conflict of interest.Estrogen treatment could prove valuable in tackling high blood pressure (hypertension) in postmenopausal women. The rats were randomly assigned to three groups (n = 4 per group): a group subjected to sham surgery (sham group), a group subjected to ovariectomy (OVX; OVX group), and a group subjected to ovariectomy with daily abdominal subcutaneous injections of 200 µg of EMouse cortical collecting duct (mpkCCDc14) cells (provided by Alain Vandewalle and Marcelle Bens, INSERM, Paris, France) were cultured in flasks (passage 30-40) in defined media consisting of equal volumes of DMEM and F12 containing 2% FBS, hormones and other nutrients, as previously describedLysates harvested from mpkCCDc14 cells were resolved by 10% SDS-PAGE on gels that contained 50 mM Tris-HCl (pH 6.8), 10% ammonium persulfate, 30% Acr-Bis, 10% SDS, and 1% TEMED. Unable to load your collection due to an error The sequences of the primers used in the qPCR were as follows: α-ENaC, forward, 5′-TGTGTCCAGCTACAAACCAATG-3′ and reverse, 5′-CATCATGCCCACTTCGTAACA-3′; derlin-1, forward, 5′-CATCACGCGCTACTGGTTTG-3′ and reverse, 5′-GAACGGCCTCCATATCTGGAA-3′; and arpppo, forward, 5′-GAAACTGCTGCCTCACATCCG-3′ and reverse, 5′-GCTGGCACAGTGACCTCACACG-3′.Statistical analyses were performed using SPSS 13.0 software. OVX rat kidneys showed increased α-ENaC expression but decreased derlin-1 expression. Estrogen and progesterone modulate both plasma volume (PV) and peripheral vascular resistance (PVR). Differences in the various parameters among the groups were evaluated by ANOVA with post hoc comparisons tests. and X.L. There is no clear mechanism for sodium and water retention caused by NSAIDs but we have to know that Long-term administration of NSAIDs has resulted in renal papillary necrosis and other renal injury. A., Stokes, J.
& Johnson, J. P. Regulation of the epithelial sodium channel by membrane trafficking. A 10 μl mixture containing 1000 ng of RNA and a set of gene-specific primers were subjected to real-time PCR using an ABI StepOnePlus System (Bio-Rad). You can also search for this author in 2017 Jul;171:236-246. doi: 10.1016/j.jsbmb.2017.04.004. A study looking at various doses of testosterone enanthate in older men revealed nearly half experienced leg edema in the groups provided with supraphysiologic testosterone (300 and 600 milligrams per week of testosterone enanthate). FoxO1 inhibits transcription and membrane trafficking of epithelial Na+ channel.
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