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For sirolimus (and tacrolimus) the effective therapeutic concentrations can be less than 5 microgram/L, so HPLC-mass spectrometry provides the most specific and accurate results.Mycophenolate is the prodrug of mycophenolic acid which inhibits purine synthesis by inhibiting inosine monophosphate dehydrogenase. https://www.youtube.com/playlist?list=PL6Uv55zOgsoDwBd4qahYq6o_QhFHXPRyd
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It binds with cyclophilin to inhibit calcineurin.Cyclosporin has a narrow therapeutic range with large inter-and intra-subject pharmacokinetic variability. Cyclosporine, azathioprine, corticosteroids, FK506 (tacrolimus), and RS61443 (mycophenolate mofetil) have been approved by the Food and Drug Administration, and the clinical efficacy of rapamycin (sirolimus), mizoribine, 15-deoxyspergualin, and leflunomide is being explored. By suppressing the T-lymphocyte cell lines, they prevent their involvement in the immune response.
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